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ACH (Acetylcholine) ELISA Kit

Product Specifications

Abbreviation

ACH

Cross Reactivity

This kit recognizes Universal ACH in samples.No significant cross-reactivity or interference between Universal ACH and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Signal transduction; Immunology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ACH. During the reaction, Universal ACH in the sample or standard competes with a fixed amount of Universal ACH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ACH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ACH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/5wzp00

Product MSDS

https://789.bio/eb/48a94K

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100808

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/

Citation 03

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03896f

Citation 04

https://www.mdpi.com/1172448

Citation 05

https://www.sciencedirect.com/science/article/pii/S1319562X21005830

Citation 06

https://www.sciencedirect.com/science/article/pii/S0045653518321131

Citation 07

https://www.mdpi.com/1473434

Citation 08

https://www.mdpi.com/2075-1729/12/9/1406

Citation 09

https://www.mdpi.com/1261728

Citation 10

https://www.liebertpub.com/doi/abs/10.1089/jmf.2018.0197

Citation 11

https://www.sciencedirect.com/science/article/pii/S0304394019302137

Citation 12

https://www.hindawi.com/journals/ecam/2021/8884243/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of ACH concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

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