5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit

Product Specifications

Abbreviation

5-HIAA

Cross Reactivity

This kit recognizes Universal 5-HIAA in samples.No significant cross-reactivity or interference between Universal 5-HIAA and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 5-HIAA. During the reaction, Universal 5-HIAA in the sample or standard competes with a fixed amount of Universal 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 5-HIAA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 5-HIAA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2152468

Citation 02

https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo01720a

Citation 03

https://www.mdpi.com/2076-3425/10/10/733

Citation 04

https://www.sciencedirect.com/science/article/pii/S0378874118344726

Citation 05

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0166966

Citation 06

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177940

Citation 07

https://www.sciencedirect.com/science/article/pii/S0091305715300678

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma and other biological fluids.

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