GnRH (Gonadotropin Releasing Hormone) ELISA Kit

Product Specifications

Abbreviation

GnRH

UniProt

P01148

Cross Reactivity

This kit recognizes Universal GnRH in samples.No significant cross-reactivity or interference between Universal GnRH and analogues was observed

Target

GNRH1; GRH; HH12; LHRH; LNRH; Luliberin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal GnRH. During the reaction, Universal GnRH in the sample or standard competes with a fixed amount of Universal GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal GnRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal GnRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S216225312100130X

Citation 02

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 03

https://www.sciencedirect.com/science/article/pii/S0269749120301469

Citation 04

https://www.mdpi.com/2073-4409/11/10/1690

Citation 05

https://www.dovepress.com/pseudoephedrine-nanoparticles-alleviate-adriamycin-induced-reproductiv-peer-reviewed-fulltext-article-IJN

Citation 06

https://www.sciencedirect.com/science/article/pii/S0147651320305376

Citation 07

https://www.sciencedirect.com/science/article/pii/S0147651320315840

Citation 08

https://www.sciencedirect.com/science/article/pii/S0753332222004504

Citation 09

https://www.sciencedirect.com/science/article/pii/S0048969719309295

Citation 10

https://www.sciencedirect.com/science/article/pii/S0269749119338801

Citation 11

https://www.sciencedirect.com/science/article/pii/S0045653519313864

Citation 12

https://www.sciencedirect.com/science/article/pii/S0024320522001989

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.

Available Sizes

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