SP (Substance P) ELISA Kit

Product Specifications

Abbreviation

UniProt

H:P20366; M:P41539; R:P06767

Cross Reactivity

This kit recognizes Universal SP in samples.No significant cross-reactivity or interference between Universal SP and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal SP. During the reaction, Universal SP in the sample or standard competes with a fixed amount of Universal SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal SP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal SP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1007/s00395-022-00968-x

Citation 02

https://www.mdpi.com/2072-6643/14/19/4083

Citation 03

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03896f

Citation 04

https://www.sciencedirect.com/science/article/pii/S0944711321004153

Citation 05

https://www.sciencedirect.com/science/article/pii/S0378874121008746

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874120332967

Citation 07

https://www.sciencedirect.com/science/article/pii/S0378874121001513

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185139/

Citation 09

https://www.frontiersin.org/articles/10.3389/fmed.2020.00179/full

Citation 10

https://www.sciencedirect.com/science/article/pii/S0006899319304202

Citation 11

https://www.sciencedirect.com/science/article/pii/S0304394020307412

Citation 12

https://www.hindawi.com/journals/ecam/2020/1471824/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids.

Available Sizes

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