MDA (Malondialdehyde) ELISA Kit

Product Specifications

Abbreviation

MDA

Cross Reactivity

This kit recognizes Universal MDA in samples.No significant cross-reactivity or interference between Universal MDA and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Signal transduction; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal MDA. During the reaction, Universal MDA in the sample or standard competes with a fixed amount of Universal MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal MDA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal MDA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S014296122200360X

Citation 02

https://link.springer.com/article/10.1007/s42765-022-00252-6

Citation 03

https://www.sciencedirect.com/science/article/pii/S2090123222001229

Citation 04

https://www.sciencedirect.com/science/article/pii/S2213231719315496

Citation 05

https://www.sciencedirect.com/science/article/pii/S0891584922009455

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332222010629

Citation 07

https://www.sciencedirect.com/science/article/pii/S0891584922001514

Citation 08

https://www.hindawi.com/journals/omcl/2022/9041914/

Citation 09

https://www.hindawi.com/journals/omcl/2022/7288729/

Citation 10

https://www.mdpi.com/1968532

Citation 11

https://www.sciencedirect.com/science/article/pii/S0147651322000240

Citation 12

https://www.mdpi.com/1188916

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of MDA concentrations in serum, plasma and other biological fluids.

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