Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

IP3 (Inositol Triphosphate) ELISA Kit

Product Specifications

Abbreviation

IP3

Cross Reactivity

This kit recognizes Universal IP3 in samples.No significant cross-reactivity or interference between Universal IP3 and analogues was observed

Target

Inositol trisphosphate; inositol 1;4;5-trisphosphate; triphosphoinositol; InsP3; Ins3P; IP3

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Biochemicals

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal IP3. During the reaction, Universal IP3 in the sample or standard competes with a fixed amount of Universal IP3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal IP3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal IP3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-018-0930-x

Citation 02

https://www.mdpi.com/1919214

Citation 03

https://www.nature.com/articles/s42003-021-02060-x

Citation 04

https://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqac217/6967064

Citation 05

https://www.hindawi.com/journals/omcl/2020/5281795/

Citation 06

https://febs.onlinelibrary.wiley.com/doi/abs/10.1111/febs.14972

Citation 07

https://www.sciencedirect.com/science/article/pii/S030441652200160X

Citation 08

https://www.nature.com/articles/s41598-020-67287-w

Citation 09

https://www.sciencedirect.com/science/article/pii/S0014482722000738

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of IP3 concentrations in serum, plasma and other biological fluids.

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Vimentin (Mesenchymal Cell Marker) (VIM/1937R), 0.2mg/mL
BNUB1937-500 1x 500 µL

Vimentin (Mesenchymal Cell Marker) (VIM/1937R), 0.2mg/mL

Sign In for Pricing
View Details
Ferritin, Light Chain (FTL) (Microglia Marker) (FTL/1388), CF488A conjugate, 0.1mg/mL
BNC881388-100 1x 100 µL

Ferritin, Light Chain (FTL) (Microglia Marker) (FTL/1388), CF488A conjugate, 0.1mg/mL

Sign In for Pricing
View Details
FBXW4 Polyclonal Antibody
E-AB-67953-01 60 µL

FBXW4 Polyclonal Antibody

Sign In for Pricing
View Details
FBXW4 Polyclonal Antibody
E-AB-67953-02 120 µL

FBXW4 Polyclonal Antibody

Sign In for Pricing
View Details
FBXW4 Polyclonal Antibody
E-AB-67953-03 200 µL

FBXW4 Polyclonal Antibody

Sign In for Pricing
View Details
PAX5 / BSAP (Early B-Cell Marker) (PCRP-PAX5-1B1), CF640R conjugate, 0.1mg/mL
BNC401930-100 1x 100 µL

PAX5 / BSAP (Early B-Cell Marker) (PCRP-PAX5-1B1), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details