IP3 (Inositol Triphosphate) ELISA Kit

Product Specifications

Abbreviation

IP3

Cross Reactivity

This kit recognizes Universal IP3 in samples.No significant cross-reactivity or interference between Universal IP3 and analogues was observed

Target

Inositol trisphosphate; inositol 1;4;5-trisphosphate; triphosphoinositol; InsP3; Ins3P; IP3

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Biochemicals

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal IP3. During the reaction, Universal IP3 in the sample or standard competes with a fixed amount of Universal IP3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal IP3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal IP3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-018-0930-x

Citation 02

https://www.mdpi.com/1919214

Citation 03

https://www.nature.com/articles/s42003-021-02060-x

Citation 04

https://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqac217/6967064

Citation 05

https://www.hindawi.com/journals/omcl/2020/5281795/

Citation 06

https://febs.onlinelibrary.wiley.com/doi/abs/10.1111/febs.14972

Citation 07

https://www.sciencedirect.com/science/article/pii/S030441652200160X

Citation 08

https://www.nature.com/articles/s41598-020-67287-w

Citation 09

https://www.sciencedirect.com/science/article/pii/S0014482722000738

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of IP3 concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

A64304-100UG 100 µg

RAB3C Antibody

Ask

View Details

HP217336 200 Reactions

ADAM32 Human qPCR Primer Pair (NM_145004)

Ask

View Details

ICH5015A-0.1mg 0.1 mg

Anti-Human CD137 (IB17) Antibody

Ask

View Details

RR207142 10 µg

Aldh3a1 (NM_031972) Rat Tagged ORF Clone

Ask

View Details

MBS7614460-01 0.05 mg

Recombinant Mouse ANP

Ask

View Details

MBS7614460-02 0.2 mg

Recombinant Mouse ANP

Ask

View Details