CAMP (Cyclic adenosine monophosphate) ELISA Kit

Product Specifications

Abbreviation

CAMP

Cross Reactivity

This kit recognizes Universal cAMP in samples.No significant cross-reactivity or interference between Universal cAMP and analogues was observed

Target

Cyclic adenosine monophosphate; cAMP; cyclic AMP;3'-5'-cyclic adenosine monophosphate

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal cAMP. During the reaction, Universal cAMP in the sample or standard competes with a fixed amount of Universal cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41467-022-33824-6

Citation 02

https://www.nature.com/articles/s41419-022-04562-w

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332222014846

Citation 04

https://www.mdpi.com/1745064

Citation 05

https://www.mdpi.com/article/10.3390/cells11182771

Citation 06

https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000872&rev=1

Citation 07

https://www.sciencedirect.com/science/article/pii/S0223523422005748

Citation 08

https://pubs.rsc.org/en/content/articlehtml/2020/bm/c9bm01352k

Citation 09

https://www.sciencedirect.com/science/article/pii/S1226845322000616

Citation 10

https://www.mdpi.com/1949936

Citation 11

https://www.mdpi.com/1422-0067/23/22/14399

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411812/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.

Available Sizes

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