LXA4 (Lipoxin A4) ELISA Kit

Product Specifications

Abbreviation

LXA4

Cross Reactivity

This kit recognizes Universal LXA4 in samples.No significant cross-reactivity or interference between Universal LXA4 and analogues was observed

Target

ASPIRIN-TRIGGERED LXA4; LXA4-15-EPI; LIPOXIN A4;15-EPI

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell Biology; Immunology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LXA4. During the reaction, Universal LXA4 in the sample or standard competes with a fixed amount of Universal LXA4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LXA4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LXA4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1186/s12951-022-01652-x

Citation 02

https://www.sciencedirect.com/science/article/pii/S0147651321008691

Citation 03

https://www.frontiersin.org/articles/10.3389/fimmu.2018.00194/full

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcpe.13173

Citation 05

https://www.sciencedirect.com/science/article/pii/S1567576921004215

Citation 06

https://www.sciencedirect.com/science/article/pii/S1567576922000704

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of LXA4 concentrations in serum, plasma and other biological fluids.

Available Sizes

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