AA (Arachidonic Acid) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Universal AA in samples.No significant cross-reactivity or interference between Universal AA and analogues was observed

Target

ARA

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal AA. During the reaction, Universal AA in the sample or standard competes with a fixed amount of Universal AA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal AA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal AA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202203995

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8421647/

Citation 03

https://www.nature.com/articles/s41389-023-00451-9

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542631/

Citation 05

https://www.frontiersin.org/articles/10.3389/fendo.2021.652807/full

Citation 06

https://europepmc.org/article/pmc/pmc8908030?javascript_support=no

Citation 07

https://www.nature.com/articles/s41420-021-00574-1

Citation 08

https://www.sciencedirect.com/science/article/pii/S2090123218301334

Citation 09

https://www.sciencedirect.com/science/article/pii/S2090123218300158

Citation 10

https://www.mdpi.com/2076-393X/8/4/682

Citation 11

https://www.sciencedirect.com/science/article/pii/S0041010116302744

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of AA concentrations in serum, plasma and other biological fluids.

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