DA (Dopamine) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Universal DA in samples.No significant cross-reactivity or interference between Universal DA and analogues was observed

Target

2- (3;4-dihydroxyphenyl) ethylamine;3;4-dihydroxyphenethylamine;3-hydroxytyramine; DA; Intropin; Revivan; Oxytyramine

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DA. During the reaction, Universal DA in the sample or standard competes with a fixed amount of Universal DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubmed.ncbi.nlm.nih.gov/34964929/

Citation 02

https://www.nature.com/articles/s41419-022-04562-w

Citation 03

https://www.mdpi.com/article/10.3390/antiox11071361

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332222015037

Citation 05

https://www.sciencedirect.com/science/article/pii/S0753332222008265

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202200481

Citation 07

https://www.sciencedirect.com/science/article/pii/S0925400519315473

Citation 08

https://www.mdpi.com/1172448

Citation 09

https://www.mdpi.com/1422-0067/19/8/2241

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/

Citation 11

https://www.mdpi.com/1449146

Citation 12

https://www.nature.com/articles/s41598-022-21054-1

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of DA concentrations in serum, plasma and other biological fluids.

Available Sizes

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