8-epi-PGF2α (8-Epi-Prostaglandin F2 Alpha) ELISA Kit

Product Specifications

Abbreviation

8-epi-PGF2α

Cross Reactivity

This kit recognizes Universal 8-epi-PGF2α in samples.No significant cross-reactivity or interference between Universal 8-epi-PGF2α and analogues was observed

Target

15-F2t-IsoP;8-iso-PGF2a;8-isoprostane;8-iso-PG;8-iso Prostaglandin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction; Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-epi-PGF2α. During the reaction, Universal 8-epi-PGF2α in the sample or standard competes with a fixed amount of Universal 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1007/s13167-021-00258-x

Citation 02

https://www.sciencedirect.com/science/article/pii/S0939641122002995

Citation 03

https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-019-1045-7

Citation 04

https://www.sciencedirect.com/science/article/pii/S0360132320308489

Citation 05

https://www.mdpi.com/2072-6643/12/6/1877

Citation 06

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265717

Citation 07

https://www.tandfonline.com/doi/abs/10.3402/fnr.v60.30209

Citation 08

https://www.sciencedirect.com/science/article/pii/S0024320519303339

Citation 09

https://www.sciencedirect.com/science/article/pii/S0024320517305441

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/10715762.2019.1685668

Citation 11

https://www.sciencedirect.com/science/article/pii/S0197457222002208

Citation 12

https://www.hindawi.com/journals/ecam/2021/8367802/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

Available Sizes

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