3-NT (3-Nitrotyrosine) ELISA Kit

Product Specifications

Abbreviation

3-NT

Cross Reactivity

This kit recognizes Universal 3-NT in samples.No significant cross-reactivity or interference between Universal 3-NT and analogues was observed

Target

3-Nitro-L-Tyrosine; Nitrotyrosine

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 3-NT. During the reaction, Universal 3-NT in the sample or standard competes with a fixed amount of Universal 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0269749122018188

Citation 02

https://www.sciencedirect.com/science/article/pii/S002432052200755X

Citation 03

https://www.mdpi.com/1265596

Citation 04

https://www.hindawi.com/journals/omcl/2021/6628957/

Citation 05

https://pubmed.ncbi.nlm.nih.gov/34970151/?utm_source=gquery

Citation 06

https://www.mdpi.com/2218-1989/13/1/49

Citation 07

https://www.hindawi.com/journals/omcl/2020/7106525/

Citation 08

https://www.nature.com/articles/s41598-021-91256-6

Citation 09

https://www.sciencedirect.com/science/article/pii/S0003986118306684

Citation 10

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174542

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.12844

Citation 12

https://www.sciencedirect.com/science/article/pii/S016801022300038X

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.

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