PGE2 (Prostaglandin E2) ELISA Kit

Product Specifications

Abbreviation

PGE2

Cross Reactivity

This kit recognizes Universal PGE2 in samples.No significant cross-reactivity or interference between Universal PGE2 and analogues was observed

Target

PG-E2; Dinoprostone

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal PGE2. During the reaction, Universal PGE2 in the sample or standard competes with a fixed amount of Universal PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1550413122004612

Citation 02

https://immunology.sciencemag.org/content/4/36/eaau6426.abstract

Citation 03

https://www.sciencedirect.com/science/article/pii/S0144861722009377

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254128/

Citation 05

https://academic.oup.com/ecco-jcc/article-abstract/9/9/708/346040

Citation 06

https://elifesciences.org/articles/54523

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332222010204

Citation 08

https://www.sciencedirect.com/science/article/pii/S0891584921000058

Citation 09

https://www.frontiersin.org/articles/10.3389/fimmu.2021.641874/full

Citation 10

https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb01389d

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352634/

Citation 12

https://www.sciencedirect.com/science/article/pii/S0944711322004391

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids.

Available Sizes

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