OT (Oxytocin) ELISA Kit

Product Specifications

Abbreviation

UniProt

H:P01178; M:P35454; R:P01179

Cross Reactivity

This kit recognizes Universal OT in samples.No significant cross-reactivity or interference between Universal OT and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Biochemicals; Signal Transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal OT. During the reaction, Universal OT in the sample or standard competes with a fixed amount of Universal OT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal OT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal OT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acsami.2c00007

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acschemneuro.2c00124

Citation 04

https://journals.sagepub.com/doi/abs/10.1177/0269881119826783

Citation 05

https://www.nature.com/articles/s41598-019-39349-1

Citation 06

https://www.sciencedirect.com/science/article/pii/S0166432818300093

Citation 07

https://www.sciencedirect.com/science/article/pii/S0166432822003503

Citation 08

https://www.sciencedirect.com/science/article/pii/S0091305718304258

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958212/

Citation 10

https://www.jstage.jst.go.jp/article/bpb/advpub/0/advpub_b21-00247/_article/-char/ja/

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052919/

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711252/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of OT concentrations in serum, plasma and other biological fluids.

Available Sizes

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