GSH (Glutathione) ELISA Kit

Product Specifications

Abbreviation

GSH

Cross Reactivity

This kit recognizes Universal GSH in samples.No significant cross-reactivity or interference between Universal GSH and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction; Cell Biology; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal GSH. During the reaction, Universal GSH in the sample or standard competes with a fixed amount of Universal GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal GSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal GSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 μg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 μg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2090123222001229

Citation 02

https://www.sciencedirect.com/science/article/pii/S0891584922009455

Citation 03

https://www.hindawi.com/journals/omcl/2022/9041914/

Citation 04

https://www.mdpi.com/1968532

Citation 05

https://link.springer.com/article/10.1007/s00204-022-03431-x

Citation 06

https://www.mdpi.com/2076-3921/10/5/746

Citation 07

https://www.sciencedirect.com/science/article/pii/S075333222200614X

Citation 08

https://pubmed.ncbi.nlm.nih.gov/34776947/

Citation 09

https://www.mdpi.com/978204

Citation 10

https://www.sciencedirect.com/science/article/pii/S1567576922010931

Citation 11

https://www.sciencedirect.com/science/article/pii/S0891584919312572

Citation 12

https://www.mdpi.com/2038768

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of GSH concentrations in serum, plasma and other biological fluids.

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