25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit

Product Specifications

Abbreviation

25-HVD3

Cross Reactivity

This kit recognizes Universal 25-HVD3 in samples.No significant cross-reactivity or interference between Universal 25-HVD3 and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Metabolism; Developmental biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 25-HVD3. During the reaction, Universal 25-HVD3 in the sample or standard competes with a fixed amount of Universal 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.13-200 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.88 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0006295222003781

Citation 02

https://link.springer.com/article/10.1007/s00223-022-00975-z

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acsapm.9b00179

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332220306417

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039685/

Citation 06

https://www.frontiersin.org/articles/10.3389/fnins.2018.00141/full?report=reader

Citation 07

https://www.sciencedirect.com/science/article/pii/S0304394022002865

Citation 08

https://www.sciencedirect.com/science/article/pii/S0271531718314490

Citation 09

https://www.degruyter.com/document/doi/10.1515/jpem-2018-0554/html

Citation 10

https://www.scielo.br/scielo.php?pid=S0004-282X2019001200848&script=sci_arttext

Citation 11

https://journals.sagepub.com/doi/abs/10.1177/0300060519896892

Citation 12

https://www.degruyter.com/document/doi/10.1515/tjb-2020-0305/html

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of 25-HVD3 concentrations in serum, plasma and other biological fluids.

Available Sizes

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