FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit

Product Specifications

Abbreviation

FA; VB9

Cross Reactivity

This kit recognizes Universal FA/VB9 in samples.No significant cross-reactivity or interference between Universal FA/VB9 and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell Biology; Metabolism; Immunology; Developmental biology; Biochemicals

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal FA/VB9. During the reaction, Universal FA/VB9 in the sample or standard competes with a fixed amount of Universal FA/VB9 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal FA/VB9. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal FA/VB9 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://academic.oup.com/ecco-jcc/article-abstract/9/9/708/346040

Citation 02

https://pubs.rsc.org/--/content/articlehtml/2016/nr/c6nr00946h

Citation 03

https://www.hindawi.com/journals/omcl/2022/9171684/

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8529044/

Citation 05

https://www.nature.com/articles/s41598-017-01732-1

Citation 06

https://www.karger.com/Article/Abstract/517911

Citation 07

https://europepmc.org/article/med/32498709?javascript_support=no

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689552/

Citation 09

https://www.tandfonline.com/doi/abs/10.1080/08039488.2019.1582697

Citation 10

https://www.sciencedirect.com/science/article/pii/S0044848614004025

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1111/anu.12275

Citation 12

https://www.ajol.info/index.php/ahs/article/view/195031

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of FA/VB9 concentrations in serum, plasma and other biological fluids.

Available Sizes

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