QuicKey Pro Rat ApoH (Apolipoprotein H) ELISA Kit
Product Specifications
Abbreviation
ApoH
UniProt
P26644
Cross Reactivity
This kit recognizes ApoH in samples.No significant cross-reactivity or interference between ApoH and analogues was observed.
Target
Apolipoprotein H; APOH; B2G1; B2GP1; BG; Beta-2-glycoprotein I; Apo-H; Anticardiolipin cofactor; Activated protein C-binding protein
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cell Biology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoH. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ApoH are added to the micro ELISA plate wells. Rat ApoH in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ApoH.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ApoH. The concentration of Rat ApoH in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
1 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,6 months
Detection Instrument
Microplate reader
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat ApoH concentrations in serum, plasma and other biological fluids.
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