SSB IgG (Human) ELISA Kit

Sjogren syndrome (SS)B/La are autoantigens that are a type of extractable nuclear antigen (ENA). They are 48 kDa proteins that are associated with RNA polymerase III newly synthesized products. Studies have demonstrated that SSB/La plays a role in the initiation and termination of RNA Polymerase III transcription. SSB/La binds to the polypyrimidine tracts at the 3’ end of the transcripts via RNA Polymerase III termination signal and stabilizes these tracts. SSB/La autoantibodies are commonly detected in the sera of patients suffering from systemic lupus erythematosus (SLE) and Sjögren's syndrome. BioVision’s SSB IgG (Human) ELISA kit quantitatively measures SSB IgG antibodies in human serum and plasma samples. Samples, calibrator, and controls are added to the wells pre-coated with SSB antigen. IgG antibodies to SSB, if present in the samples, will bind to the SSB antigen. The wells are then washed with Wash Buffer, followed by incubation with the enzyme conjugate. After incubation, any unattached conjugates are washed off by Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of IgG antibodies to SSB present in the sample.