Recombinant Human Fibroblast Growth Factor- acidic (rHu aFGF)
Recombinant Human Fibroblast Growth Factor- acidic (rHu aFGF)
Product Specifications
Product Name Alternative
FGF-1, ECGF, HBGF-1
UniProt
P05230
Source
Human
Field of Research
Cardiovascular Research; Stem Cell & Developmental Biology
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 106 IU/mg in the presence of 10 μg/ml of heparin.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Molecular Weight
Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
Storage Conditions
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Notes
For research use only.
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