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Recombinant Human Fibroblast Growth Factor- acidic (rHu aFGF)

Recombinant Human Fibroblast Growth Factor- acidic (rHu aFGF)

Product Specifications

Product Name Alternative

FGF-1, ECGF, HBGF-1

UniProt

P05230

Source

Human

Field of Research

Cardiovascular Research; Stem Cell & Developmental Biology

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 106 IU/mg in the presence of 10 μg/ml of heparin.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight

Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

Notes

For research use only.

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