Recombinant Human Fibroblast Growth Factor-basic (rHu bFGF)

Product Specifications

Product Name Alternative

FGF-2, HBGF-2

UniProt

P09038

Source

Human

Field of Research

Neuroscience; Cardiovascular Research; Stem Cell & Developmental Biology

Purity

> 96 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 107 IU/mg.

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.

Molecular Weight

Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.