Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag
Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (NP1-V52H3) is expressed from human 293 cells (HEK293) . It contains AA Met 1 - Asn 498 (Accession # EPI2224781, GISAID) .
Product Specifications
Background
Influenza, commonly known as ‘the flu’, is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses.Influenza viral nucleoprotein (NP) is highly conserved and the most abundant non-enzymatic viral protein in infected cells. NP is a key component of the viral ribonucleoproteins (vRNPs) complex, and its recognized functions include, but are not limited to, binding to RNA and oligomerizing for the vRNP complex, undergoing intracellular trafficking, and participating in the switch from mRNA transcription to vRNA replication.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.1 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
EPI2224781
Expression Region
Met 1 - Asn 498
Host
HEK293
Target
Nucleocapsid protein/NP (Influenza Virus)
Conjugation
Unconjugated
Tag
C-10xHis
Source
Influenza A virus (Wisconsin/67/2022)
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V52H3) at 1 μg/mL (100 μL/well) can bind Influenza A Nucleoprotein Antibody, Mouse MAb with a linear range of 0.05-1.56 ng/mL (QC tested).
Format
Powder
Buffer
0.2 M Arginine, PBS, pH7.4
Additives
Trehalose
Molecular Weight
58.1 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/NP1-V52H3.pdf
Species
Influenza A virus (Wisconsin/67/2022)
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