Anti-Bacterial Transglutaminase Antibody

Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Immunoblotting: use at 1-10ug/mL. A band of ~38kDa is detected <br><br>ELISA: use at 1-5ug/mL with Streptomyces Tgase on the solid phase. <br><br>These are recommended concentrations. <br><br>Enduser should determine optimal concentrations for their applications.

This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Transglutaminases (Tgases) are multifunctional enzymes that are widely distributed among mammals, inverte- brates and plants. Streptomyces mobaraensis Tgase is one of the most useful Tgases due to its rather broad substrate specificity and independence of Ca2+. The role of TGase in the life- cycle of Streptomycetes is not fully understood, but it is likely that it functions in protein cross-linking that fortifies the aerial cell wall and the spore envelope. The protein structures of the known bacterial Tgases are quite different from mammalian Tgases in that they lack sequence homology and have smaller molecular masses. Tgase from S. mobaraensis is secreted as a 42.5- kDa precursor protein which is then processed by the removal of a 45 amino-acid N-terminal peptide.