Recombinant Human Tumor Necrosis Factor-a (rHu TNF-a)
Recombinant Human Tumor Necrosis Factor-a (rHu TNF-a)
Product Specifications
Product Name Alternative
Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin
UniProt
P01375
Source
Human
Field of Research
Cancer Biology; Immunology & Inflammation
Purity
> 98 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 107 IU/mg in the presence of actinomycin D.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 10 mM Nacl, pH 7.0.
Molecular Weight
Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.
Storage Conditions
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Notes
For research use only.
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