EDTA-AM

EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) is the membrane-permeant form of the metal chelator EDTA (HY-Y0682) . Live cells passively load EDTA-AM by incubating with EDTA-AM. Once internalized, cytoplasmic esterase decomposes AM esters, releasing the active ligand EDTA, which isolates metal ions within the cell. EDTA-AM induces an arrest of mitotic progression and chromosome decondensation[1][2].

Product Specifications

CAS Number

[162303-59-5]

Product Name Alternative

EDTA acetoxymethyl ester; Ethylenediaminetetraacetic acid acetoxymethyl ester

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents; Mitosis

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/edta-am.html

Purity

98.0

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=C(C)OCOC(CN(CCN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O

Molecular Formula

C22H32N2O16

Molecular Weight

580.49

Precautions

H302, H315, H319, H335

References & Citations

[1]Watkins CS, et.al. Effects on K+ currents in rat cerebellar granule neurones of a membrane-permeable analogue of the calcium chelator BAPTA. Br J Pharmacol. 1996 Aug;118 (7) :1772-8. |[2]Maeshima K, et.al. A Transient Rise in Free Mg2+Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28 (3) :444-451.e6.