What sizes are available for Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF-α)?

Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF-α) is available in the following size(s): 1 mg, 10 µg, 50 µg.

How many pack sizes does Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF-α) come in?

Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF-α) is available in 3 different pack sizes.

Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF-α)

Q: What is the catalog number for Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF-α)?

The catalog number for Recombinant Human Tumor Necrosis Factor-alpha (rHuTNF-α) is orb1495075.

Tumor necrosis factor alpha (TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Product Specifications

Source

Escherichia coli.

Field of Research

Cancer Biology; Immunology & Inflammation

Endotoxin

Less than 1EU/mg of rHuTNF-α as determined by LAL method.

Purity

>95% by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The Specific Activity is ≥2.0 x 107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.

Form

Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.0.

Molecular Weight

Approximately 17.5 kDa. The recombinant protein preparation is a mixture of a 158 amino acid residue form containing an N-terminal methionine and a 157 amino acid residue form with the sequence of mature human TNF-α.

Storage Conditions

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Preservative

Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.0.

AA Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVVPSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL