What is the catalog number for Recombinant Rat Epidermal Growth Factor (rRtEGF)?

The catalog number for Recombinant Rat Epidermal Growth Factor (rRtEGF) is orb1495058.

What sizes are available for Recombinant Rat Epidermal Growth Factor (rRtEGF)?

Recombinant Rat Epidermal Growth Factor (rRtEGF) is available in the following size(s): 1 mg, 20 µg, 100 µg.

How many pack sizes does Recombinant Rat Epidermal Growth Factor (rRtEGF) come in?

Recombinant Rat Epidermal Growth Factor (rRtEGF) is available in 3 different pack sizes.

Is Recombinant Rat Epidermal Growth Factor (rRtEGF) in stock?

Recombinant Rat Epidermal Growth Factor (rRtEGF) is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant Rat Epidermal Growth Factor (rRtEGF)

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor) .

Product Specifications

Source

Escherichia coli.

Field of Research

Stem Cell & Developmental Biology

Endotoxin

Less than 1EU/mg of rRtEGF as determined by LAL method.

Purity

97% by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is 1 x 107 units/mg.

Form

Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.

Molecular Weight

6.2 kDa, a single non-glycosylated polypeptide chain containing 54 amino acids, including 3 intramolecular disulfide-bonds.

Storage Conditions

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.