What is the catalog number for Recombinant MurineInterleukin-11 (rMuIL-11)?

The catalog number for Recombinant MurineInterleukin-11 (rMuIL-11) is orb1495015.

What sizes are available for Recombinant MurineInterleukin-11 (rMuIL-11)?

Recombinant MurineInterleukin-11 (rMuIL-11) is available in the following size(s): 1 mg, 2 µg, 10 µg.

How many pack sizes does Recombinant MurineInterleukin-11 (rMuIL-11) come in?

Recombinant MurineInterleukin-11 (rMuIL-11) is available in 3 different pack sizes.

Is Recombinant MurineInterleukin-11 (rMuIL-11) in stock?

Recombinant MurineInterleukin-11 (rMuIL-11) is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant MurineInterleukin-11 (rMuIL-11)

Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T11. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell.

Product Specifications

Source

Escherichia coli.

Endotoxin

Less than 1EU/mg of rmIL-11 as determined by LAL method.

Purity

>97% by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine T11 was found to be less than 2.0 ng/ml, corresponding to a specific activity of >5x105 IU/mg.

Form

Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.

Molecular Weight

Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Preservative

Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.

AA Sequence

MPGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDHSLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLKTLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGSIRAAHAILGG LHLTLDWAVR GLLLLKTRL