Recombinant Rat Interleukin-10 (rRtIL-10)
Interleukin-10 also known as cytokine synthesis inhibitory factor (CSIF), is coded by IL10 gene on chromosome 13 in rat. the charter member of the IL 10 family of α helical cytokines that also includes IL19, IL20, IL22, and IL24 (1, 2) . IL10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells.
Product Specifications
Source
Escherichia coli.
Field of Research
Immunology & Inflammation
Endotoxin
Less than 1EU/μg of rRtIL-10 as determined by LAL method.
Purity
>97% by SDS-PAGE and HPLC analyses.
Bioactivity
Measured in a cell proliferation assay using MC/92 mouse mast cells. The ED50 for this effect is typically 2-12ng/mL.
Form
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Molecular Weight
Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acid residues.
Storage Conditions
Notes
For research use only.
Applications Notes
Preservative
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
AA Sequence
Available Sizes
Frequently Asked Questions
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