Recombinant Human Eotaxin-3/CCL26 (rHuEotaxin-3/CCL26)
Eotaxin3, also named CCL26 or SCYA26, is a novel human CC chemokine coded by CXCL26 gene at chromosome 7 in human. Recombinant Eotaxin3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin3/CCL26 is chemotactic for eosinophils and PHAactivated T cells. Eotaxin3/CCL26 induces calcium flux in eosinophils as well as in CCR3 transfected cells. Eotaxin3/CCL26 has also been shown to crossdesensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3 transfected cell line.
Product Specifications
Source
Escherichia coli.
Field of Research
Immunology & Inflammation
Endotoxin
Less than 1EU/μg of rHuEotaxin-3/CCL26 as determined by LAL method.
Purity
>97% by SDS-PAGE and HPLC analyses.
Bioactivity
Measured by its ability to chemoattract mouse CCR3 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 0.3-0.5μg/mL.
Form
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Molecular Weight
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 71 amino acid residues.
Storage Conditions
Notes
For research use only.
Applications Notes
Preservative
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
AA Sequence
TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L
Available Sizes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD59 (heavy chain of Protectin) (BRA-10G), CF594 conjugate, 0.1mg/mL
Eosinophil Peroxidase (AHE-1), CF405S conjugate, 0.1mg/mL
TAG-72 / CA72.4 (B72.3), CF740 conjugate, 0.1mg/mL
