RecombinantEGF, Human

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues.

Product Specifications

Product Name Alternative

Epidermal Growth Factor

Source

Escherichia coli.

Endotoxin

Less than 0.1ng/μg (1 EU/μg) of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test.

Purity

Greater than 95% as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Bioactivity

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0x10ˆ5 IU/ mg.

Form

Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer.

Molecular Weight

6.0 kDa+/-10% determined by reduced SDS-PAGE

Storage Conditions

Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

Notes

For research use only.

Applications Notes

It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.