RecombinantGM-CSF, Mouse

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine and other immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor that activates effector functions of granulocytes, monocytes/macrophages and eosinophils.Recombinant Mouse GM-CSF produced in E.coli is a single non-glycosylated polypeptide chain containing 125 amino acids. A fully biologically active molecule, rmGM-CSF has a molecular mass of 14.3 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Product Specifications

Product Name Alternative

Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim

Source

Escherichia coli.

Field of Research

Immunology & Inflammation

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 98% as analyzed by SDS-PAGE&HPLC.

Bioactivity

ED50 2 x 10ˆ8 units/mg.

Form

Lyophilized after extensive dialysis against PBS.

Molecular Weight

14.3 kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant Mouse GM-CSF remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse GM-CSF should be stable up to 1 week at 4°C or up to 3 months at -20°C.