RecombinantIL-6, Human

Interleukin-6 (IL-6, also known as IFN-β2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells.Recombinant human Interleukin-6 (rhIL-6) produced in E.coli is a single non-glycosylated polypeptide chain containing 185 amino acids. A fully biologically active molecule, rhIL-6 has a molecular mass of 20.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Product Specifications

Product Name Alternative

Interleukin-6, IL6

Source

Escherichia coli.

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE analysis.

Bioactivity

ED50 1 x 10ˆ7 units/mg.

Form

Lyophilized after extensive dialysis against 1xPBS.

Molecular Weight

20.9 kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Notes

For research use only.

Applications Notes

Reconstituted in ddH2O at 100 μg/ml.

Preservative

Lyophilized after extensive dialysis against 1xPBS.

AA Sequence

MPVPPGEDS KDVAAPHRQP LTSSERIDKQ IRYILDGISA LRKETCNKSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEETCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PDPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSSL RALRQM