RecombinantNANOG-TAT, Human
NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E.coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Specifications
Product Name Alternative
NANOG
Source
Escherichia coli.
Field of Research
Signal Transduction
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Form
Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH 7.4.
Molecular Weight
36.2 kDa, analyzed by reducing SDS-PAGE.
Storage Conditions
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O or PBS at 100 μg/ml.
Preservative
Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH 7.4.
AA Sequence
Available Sizes
Frequently Asked Questions
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