RecombinantPDGF-BB, Mouse
Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, development of human skeleton, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rmPDGF-BB has a molecular mass of 24.7 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Specifications
Product Name Alternative
PDGF
Source
Escherichia coli.
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE analysis.
Bioactivity
ED50 4 x 10ˆ5 units/mg.
Form
Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0.
Molecular Weight
24.7 kDa, observed by non-reducing SDS-PAGE.
Storage Conditions
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/mL.
Preservative
Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0.
AA Sequence
MSLGSLAAA EPAVIAECKT RTEVFQISRN LIDRTNANFL VWPPCVEVQR CSGCCNNRNV QCRASQVQMR PVQVRKIEIV RKKPIFKKAT VTLEDHLACK CETIVTPRPV T
Available Sizes
Frequently Asked Questions
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