RecombinantShh (C25II), Mouse

Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) . They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical) . Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant mouse Sonic Hedgehog (C25II) (rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rmShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Product Specifications

Product Name Alternative

SHH, HHG-1

Source

Escherichia coli.

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Bioactivity

ED50 500 units/mg.

Form

Lyophilized after extensive dialysis against PBS.

Molecular Weight

19.7 kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant mouse Sonic Hedgehog (C25II) (rmShh) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmShh should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Notes

For research use only.

Applications Notes

Reconstituted in ddH2O at 100 μg/ml.

Preservative

Lyophilized after extensive dialysis against PBS.

AA Sequence

IIG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT LGASGRYEGK ITRNSERFKE LTPNYNPDII FKDEENTGAD RLMTQRCKDK LNALAISVMN QWPGVKLRVT EGWDEDGHHS EESLHYEGRA VDITTSDRDR SKYGMLARLA VEAGFDWVYY ESKAHIHCSV KAENSVAAKS GG