RecombinantEnterokinase, His, Bovine
Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Product Specifications
Product Name Alternative
Enteropeptidase, ENTK, PRSS7
Source
P. pastoris
Endotoxin
< 1.0 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE analyses.
Bioactivity
Form
Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
Molecular Weight
Theoretical MW: 22.7 kDa.Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O or PBS at 100 μg/ml.
Preservative
Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
AA Sequence
0
Available Sizes
Frequently Asked Questions
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