RecombinantSCF, Human (P.pastoris-expressed)
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.Recombinant human Stem Cell Factor (rhSCF) produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ~20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Product Specifications
Product Name Alternative
C-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor
Source
P. pastoris
Endotoxin
< 1.0 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE analysis.
Bioactivity
The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5x10ˆ5 IU/mg.
Form
Lyophilized after extensive dialysis against 10 mM acetic acid.
Molecular Weight
~20 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against 10 mM acetic acid.
AA Sequence
Available Sizes
Frequently Asked Questions
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