DT3C Protein
This DT3C Protein spans the amino acid sequence from region 33-417aa (P00588) +linker (GGGGSGGGGSGGGGS) +291-497aa (P19909) . Purity: Greater than 90% as determined by SDS-PAGE.
Product Specifications
UniProt
P19909, P00588
Expression Region
33-417aa (P00588) +linker (GGGGSGGGGSGGGGS) +291-497aa (P19909)
Tag
N-terminal 6xHis-tagged
Source
E.coli
Origin
Corynephage beta
Field of Research
Epigenetics, Non-Animal
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 90% as determined by SDS-PAGE.
Bioactivity
1After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells. The ED50 is 0.3708-0.5608 μg/ml. 2After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CT26/Human CCR8 cells. The ED50 is 0.4661-0.8614 μg/ml.
Form
Lyophilized powder
Molecular Weight
69.4 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
AA Sequence
GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKGGGGSGGGGSGGGGSIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE
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