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DT3C Protein

This DT3C Protein spans the amino acid sequence from region 33-417aa (P00588) +linker (GGGGSGGGGSGGGGS) +291-497aa (P19909) . Purity: Greater than 90% as determined by SDS-PAGE.

Product Specifications

UniProt

P19909, P00588

Expression Region

33-417aa (P00588) +linker (GGGGSGGGGSGGGGS) +291-497aa (P19909)

Tag

N-terminal 6xHis-tagged

Source

E.coli

Origin

Corynephage beta

Field of Research

Epigenetics, Non-Animal

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 90% as determined by SDS-PAGE.

Bioactivity

1After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells. The ED50 is 0.3708-0.5608 μg/ml. 2After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CT26/Human CCR8 cells. The ED50 is 0.4661-0.8614 μg/ml.

Form

Lyophilized powder

Molecular Weight

69.4 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Preservative

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

AA Sequence

GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKGGGGSGGGGSGGGGSIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE

Available Sizes

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