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Bovine FGF 21 Protein

Recombinant of bovine FGF 21 protein

Product Specifications

Product Name Alternative

Fibroblast growth factor 21, FGF-21, FGF21.

Source

Escherichia Coli

Field of Research

Stem Cell & Developmental Biology

Purity

Greater than 98.0% as determined by: (a) Analysis by Gel Filtration. (b) Analysis by SDS-PAGE.

Form

Sterile Filtered white lyophilized powder.

Solubility

It is recommended to reconstitute the lyophilized Bovine FGF-21 in sterile water or 0.4% NaHCO3, not less than 100μg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Storage Conditions

Stability: Lyophilized FGF-21 Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles

Notes

For research use only.

Applications Notes

Protein content: Bovine FGF-21 quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis by RP-HPLC, using a standard solution of FGF-21 Recombinant as a Reference Standard

Preservative

The protein was lyophilized from a concentrated (0.8 mg/ml) solution with 0.4 mg/ml of NaHCO3, pH 8.

AA Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-His-Pro-Ile-Pro

Available Sizes

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