Bovine FGF 21 Protein
Recombinant of bovine FGF 21 protein
Product Specifications
Product Name Alternative
Fibroblast growth factor 21, FGF-21, FGF21.
Source
Escherichia Coli
Field of Research
Stem Cell & Developmental Biology
Purity
Greater than 98.0% as determined by: (a) Analysis by Gel Filtration. (b) Analysis by SDS-PAGE.
Form
Sterile Filtered white lyophilized powder.
Solubility
It is recommended to reconstitute the lyophilized Bovine FGF-21 in sterile water or 0.4% NaHCO3, not less than 100μg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Storage Conditions
Stability: Lyophilized FGF-21 Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Protein content: Bovine FGF-21 quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis by RP-HPLC, using a standard solution of FGF-21 Recombinant as a Reference Standard
Preservative
The protein was lyophilized from a concentrated (0.8 mg/ml) solution with 0.4 mg/ml of NaHCO3, pH 8.
AA Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-His-Pro-Ile-Pro
Available Sizes
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