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Human IL 7 Protein

Recombinant of human, yeast IL 7 protein

Product Specifications

Product Name Alternative

Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.

Source

Saccharomyces cerevisiae

Purity

Greater than 98.0% as determined by SDS-PAGE.

Bioactivity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is 2 x 106 units/mg.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility

It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions

Stability: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles

Notes

For research use only.

Applications Notes

Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard

Preservative

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer.

AA Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu

Available Sizes

Frequently Asked Questions

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