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Human Leptin Protein

Recombinant of human Leptin protein

Product Specifications

Product Name Alternative

OB Protein, Obesity Protein, OBS, Obesity factor.

Source

Escherichia Coli

Field of Research

Metabolism Research

Purity

Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.

Bioactivity

Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility

The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.

Storage Conditions

Stability: Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C

Notes

For research use only.

Applications Notes

Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard

Preservative

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

AA Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln

Available Sizes

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