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Leptin (PEG) Protein

Recombinant of Leptin (PEG) protein

Product Specifications

Product Name Alternative

OB Protein, Obesity Protein, OBS, Obesity factor.

Source

Escherichia Coli

Field of Research

Metabolism Research

Purity

Greater than 99.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.

Bioactivity

Pegylated Rat Leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is only slightly lower than the non-pegylated antagonist but in vivo it has profound weight reducing effect (as compared to the non-pegylated leptin), resulting mainly from reduced food intake.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility

It is recommended to reconstitute the lyophilized pegylated Rat Leptin in sterile 0.4% NaHCO3 adjusted to pH-8.5, not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions

Stability: Lyophilized Rat Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles

Notes

For research use only.

Applications Notes

Cytokines And Growth Factors

Preservative

The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.

Available Sizes

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