CAS9 Protein
Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease
Product Specifications
Product Name Alternative
CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.
Source
Escherichia Coli
Field of Research
Molecular Biology
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Sterile Filtered colorless solution.
Storage Conditions
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles
Notes
For research use only.
Applications Notes
Enzymatic Activity: 97% of PCR product digestion after 30 minutes at 37°C. Endonuclease Activity: The incubation of 20pmol of CAS9 protein with 1μg pUC19 supercoiled vector at 37°C for 4 hours leads to formation of less than 10% open circular pUC19 form as determined by gel electrophoresis. Non-specific DNAse activity: The incubation of 20pmol of CAS9 protein with 1μg of lambda DNA at 37°C for 4 hours is free of detectable DNA degradation, as visualized on agarose gel
Preservative
The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
GTR15400956 1 Vial
Lentiviral human ZBTB39 sgRNA gene knockout kit - Lentiviral human ZBTB39 sgRNA gene knockout kit (25)
Sign In for Pricing
View Details 