CAS9 Protein

Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles

Enzymatic Activity: 97% of PCR product digestion after 30 minutes at 37°C. Endonuclease Activity: The incubation of 20pmol of CAS9 protein with 1μg pUC19 supercoiled vector at 37°C for 4 hours leads to formation of less than 10% open circular pUC19 form as determined by gel electrophoresis. Non-specific DNAse activity: The incubation of 20pmol of CAS9 protein with 1μg of lambda DNA at 37°C for 4 hours is free of detectable DNA degradation, as visualized on agarose gel