Lipase A Protein
Recombinant of Lipase A protein
Product Specifications
Source
Escherichia Coli
Field of Research
Metabolism Research
Purity
Greater than 90% as determined by SDS-PAGE.
Bioactivity
580 units/mg powder.
Form
Sterile Filtered lyophilized powder.
Solubility
It is recommended to reconstitute the lyophilized Lipase-A in sterile 10%-50% DMSO, isopropyl ether, petroleum ether, ethanol, acetone and isopropanol.
Storage Conditions
Stability: Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -1°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Unit Definition: Lipase-A was assayed using p-nitrophenyl acetate (pNPA) as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Application Stability: pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether and petroleum ether, 10%-25% ethanol, acetone and isopropanol, 50% activity can be kept in 50% acetone and isopropanol, no activity detected in 50% ethanol
Preservative
The protein was lyophilized without additives.
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