Lysostaphin Protein

Recombinant Lysostaphin protein

Product Specifications

Product Name Alternative

Lysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase.

Source

Escherichia Coli

Purity

98% as determined by RP-HPLC.

Bioactivity

Assessed by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH-8, 30°C. Lysostaphin is a zinc enzyme therefore EDTA is an inhibitory factor.

Form

Sterile Filtered lyophilized powder.

Solubility

It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5 for optimal stability, which can then be further diluted.

Storage Conditions

Stability: Lyophilized Lysostaphin although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysostaphin can be stored at 4°C up to 3 weeks and for future use below -18°C.Please prevent freeze-thaw cycles

Notes

For research use only.

Applications Notes

Protein content: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference standard